Probing spermiogenesis: a digital strategy for mouse acrosome classification
نویسندگان
چکیده
منابع مشابه
Flotillin-2 is an acrosome-related protein involved in mouse spermiogenesis
SPERMATOGENESIS IS A COMPLEX PROCESS OF TERMINAL DIFFERENTIATION BY WHICH MATURE SPERMS ARE GENERATED, AND IT CAN BE DIVIDED INTO THREE PHASES: mitosis, meiosis and spermiogenesis. In a previous study, we established a series of proteomic profiles for spermatogenesis to understand the regulation of male fertility and infertility. Here, we further investigated the localization and the role of fl...
متن کاملICA1L forms BAR-domain complexes with PICK1 and is crucial for acrosome formation in spermiogenesis.
Mutations in the Pick1 gene cause globozoospermia, a male infertility disorder, in both mice and humans. PICK1 is crucial for vesicle trafficking, and its deficiency in sperm cells leads to abnormal vesicle trafficking from the Golgi to the acrosome. This eventually disrupts acrosome formation and leads to male infertility. Here, we identified ICA1L, which has sequence similarities to ICA69 (al...
متن کاملICA1L Forms BAR-domain Complexes with PICK1 and is Critical for Acrosome Formation in Spermiogenesis
Mutations of the Pick1 gene cause globozoospermia, a male infertility disorder in both mice and human. PICK1 is critical for vesicle trafficking and its deficiency in sperm cells leads to abnormal vesicle trafficking from the Golgi to acrosome. This eventually disrupts acrosome formation and leads to male infertility. We identified a novel BAR-domain binding partner of PICK1: ICA1L, which has s...
متن کاملKIFC1 is essential for acrosome formation and nuclear shaping during spermiogenesis in the lobster Procambarus clarkii
In order to study the function of kinesin-14 motor protein KIFC1 during spermatogenesis of Procambarus clarkii, the full length of kifc1 was cloned from testes cDNA using Rapid-Amplification of cDNA Ends (RACE). The deduced KIFC1 protein sequence showed the highest similarity between Procambarus clarkii and Eriocheir senensis (similarity rate as 64%). According to the results of in situ hybridi...
متن کاملAutomatic classification of the acrosome status of boar spermatozoa using digital image processing and LVQ
We consider images of boar spermatozoa obtained with an optical phase-contrast microscope. Our goal is to automatically classify single sperm cells as acrosome-intact (class 1) or acrosome-damaged (class 2). Such classification is important for the estimation of the fertilization potential of a sperm sample for artificial insemination. We segment the sperm heads and compute a feature vector for...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-03867-7